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TA : Pelin Kübra İşgör |
Topics covered:
Microfluidic systems
Droplet generation devices
Fabrication of microfluidic devices
Introduction to Elveflow pressure pump and Microqubic 3D digital microscope
Droplet generation at varying sizes
Droplet detection using via video/image processing
Experiment details:
DROPLET GENERATION EXPERIMENT
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Switch on Elveflow pump. Make sure inlet ports are closed.
Open vacuum source.
Open ESI software. Click on Calibration button. Let the system calibrate itself. Calibrate the system twice.
Switch on Microqubic 3D digital microscope.
Open Microqubic software. Choose See3Cam camera.
Under camera options adjust Exposure, set it to Auto.
Put your microfluidic chip on the stage.
Using joystick adjust focus until you observe microfluidic channels.
Connect DI and silicone oil vial to the inlets of pump.
Never let fluid to go inside pressure line.
On ESI software, click Stop All button.
Give 10 mbar pressure to the DI line. Wait until fluid comes at the edge of the precision tip.
Insert DI precision tip to the DI inlet on the microfluidic chip.
Close pressure.
Give 50 mbar pressure to the silicone oil line. Wait until fluid comes at the edge of the precision tip.
Insert DI precision tip to the DI inlet on the microfluidic chip.
Close pressure.
Go to DI inlet. Increase pressure to 20 mbar. Watch for the fluid while it is going through the microchannel. At this point you can lower the pressure, because DI should not go into the silicone oil channel. The reverse is also valid.
Go to silicone oil inlet. Increase pressure to 40 mbar. Watch for the fluid while it is going through the microchannel. At this point you can lower the pressure, because silicone oil should not go into the DI channel.
Using microscope joystick, go to the junction where droplet will be generated.
Adjust pressures accordingly until you observe droplet generation.
After droplet generation is observed, increase pressure of DI and silicone oil proportionally to observe varying size and speed droplets.
Take a note of pressure values for both DI and silicone oil.
Take a video using Microqubic software.
Use this video for image processing to determine droplet sizes (Code will be provided).
Report requirements:
The report for this part is expected to include:
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